Molecular diagnosis of Vector Mosquitoes
rDNA-ITS2 based probes for Anopheles fluviatilis 
rDNA-ITS2 region for members of Anopheles subpictus
Molecular diagnosis for filarial parasite

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Two genes specific for infective stage (L3) larvae of Wuchereria bancrofti were identified, sequenced and developed as probes for detecting vector infectivity by RT-PCR assay.
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A monoclonal antibody specific to e/s antigens of fourth stage larvae of Wuchereria bancrofti was developed which has potential application in the early diagnosis of filarial infection.
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PCR assays developed for detecting Wuchereria bancrofti and Brugia malayi infection in respective vectors were evaluated and found to be highly specific. Sensitivity-wise B. malayi PCR assay was found to be equal to the conventional technique and that of Wuchereria bancrofti was less.
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A simple, efficient, rapid and cost-effective method for the extraction of DNA from a single mf in pools of 25 mosquitoes, stable for about a year and suitable for PCR amplification yielding a species specific band of 322 bp with primers specific for Brugia malayi was developed.
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Random Amplified Fragment (RAPD) analysis of Wuchereria bancrofti from three villages showed heterogeneity among parasite population.
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The sensitivity and specificity of the day blood Immunochormatography Card Test (ICT) for bancroftian filariasis was 98.5% and 100% respectively compared to the finger prick thick smear night blood test.
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The ICT is suitable for delimitation and for spot treatment decision as the result is available within 2-5 min of testing.
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The sensitivity and specificity of Og4C3 monoclonal antibody ELISA test were 100% and 97% respectively compared to the finger prick thick smear night blood test.
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The Og4C3 kit is suitable for quantifying antigen levels for monitoring and evaluation of control programmes.
Immunoprophylactics for malarial parasites
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Two conserved antigens of Plasmodium falciparum which hold promise as vaccine candidates were expressed in E.coli.
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These were found to contain novel genes, which were conserved across different species of Plasmodium.