Paper II: Culicines and their Medical Importance

A. Lymphatic filariasis

Unit I:

  • Recent trends on the prevalence of LF in India

  • Life history of Wuchereria bancrofti and Brugia malayi

  • Parasite morphology and microfilariae periodicity

  • Biology and ecology of Culex quinquefasciatus and Mansonioides vectors

Unit II: Survey methods: Conventional – entomological – parasitological and clinical

Molecular and immunodiagnostic methods including xenomonitoring and PCR techniques for parasites and species identification

Epidemiological indices: Infection and infectivity rates, vector density, Annual biting rate, mf rate, mf density, endemicity rate, Annual infective biting rate, Transmission Intensity Index (TII)and Annual Transmission Potential (ATP).

Unit III: Chemotherapy: Selective treatment – mass drug administration – medicated salt – side reactions

Unit IV: National Filaria Control Programme in India
LF elimination strategy – Annual single dose MDA and Morbidity management measures

B. Arboviral diseases

Unit V:

Japanese encephalitis: Geographical distribution – vectors – parasite transmission cycle – risk factors – prevention and control – vaccination

Dengue and chikungunya: Distribution – vectors – transmission prevention


  • Dissection of Culex quinquefasciatus and demonstration of various filarial larval stages

  • Preparation of JSB stain

  • Night blood survey

  • Staining and examination of thick blood smears for mf

  • Demonstration of membrane filtration technique

  • Differentiation of mf and L3 of W. bancrofti and B. malayi

  • Demonstration of ICT card test

  • Demonstration of Og4C3 technique

  • Demonstration of PCR for the detection of filarial larvae in mosquitoes

Last Updated on:18/09/2019